Native Glycosylation Is Important For Biological Function and Activity of Recombinant Human Proteins
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The Relationship of Secretion and Activity of Recombinant Factor IX with N-Glycosylation
Background: Human coagulation factor IX (hFIX) is a glycoprotein with two N-glycosylation sites at the activation peptide. Since the activation peptide is removed in mature hFIX, the exact role of N-glycosylation is unclear. To investigate the role of N-glycosylation in the secretion and activity of hFIX, we inhibited N-glycosylation by tunicamycin in the stable Human Embryonic Kidney (HEK)- c...
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تاریخ انتشار 2015